Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction
نویسندگان
چکیده
منابع مشابه
A survey of error-correction methods for next-generation sequencing
UNLABELLED Error Correction is important for most next-generation sequencing applications because highly accurate sequenced reads will likely lead to higher quality results. Many techniques for error correction of sequencing data from next-gen platforms have been developed in the recent years. However, compared with the fast development of sequencing technologies, there is a lack of standardize...
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Motivation Next-generation sequencing platforms have produced huge amounts of sequence data. This is revolutionizing every aspect of genetic and genomic research. However, these sequence datasets contain quite a number of machine-induced errors-e.g. errors due to substitution can be as high as 2.5%. Existing error-correction methods are still far from perfect. In fact, more errors are sometimes...
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It is clear that the use of next generation sequencing (NGS) applied to environmental DNA is changing the way researchers conduct experiments and significantly deepening our understanding of microbial communities around the globe (Amend et al., 2010; Caporaso et al., 2011; Bik et al., 2012; Bates et al., 2013). The lower per unit cost and sheer number of sequences relative to traditional method...
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It is clear that the use of next generation sequencing (NGS) applied to environmental DNA is changing the way researchers conduct experiments and significantly deepening our understanding of microbial communities around the globe (Amend et al., 2010; Caporaso et al., 2011; Bik et al., 2012; Bates et al., 2013). The lower per unit cost and sheer number of sequences relative to traditional method...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-67290-1